Anti-Human PDK1 Antibody (SAA1680)

Description: Anti-Human PDK1 Antibody (SAA1680) [SAA1680] (RA12324) is a mouse monoclonal antibody detecting PDK1 in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: PDHK1, [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial, PDK1, Pyruvate dehydrogenase kinase isoform 1, PDH kinase 1
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: [Pyruvate dehydrogenase (acetyl-transferring)] kinase isozyme 1, mitochondrial (PDK1) is a ~49 kDa protein. Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia.
References:
1. Gudi, R. et al. (1995) The Journal of biological chemistry 270, 28989-94. PMID: 7499431
2. McFate, T. et al. (2008) The Journal of biological chemistry 283, 22700-8. PMID: 18541534
3. Hitosugi, T. et al. (2011) Molecular cell 44, 864-77. PMID: 22195962
4. Li, X. et al. (2016) Molecular cell 61, 705-719. PMID: 26942675
5. Kato, M. et al. (2007) Structure (London, England : 1993) 15, 992-1004. PMID: 17683942
Note: For research use only.