Anti-Human TLR7 Antibody (SAA1718)

Description: Anti-Human TLR7 Antibody (SAA1718) [SAA1718] (RA12362) is a mouse monoclonal antibody detecting TLR7 in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: TLR7, Toll-like receptor 7
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Toll-like receptor 7 (TLR7) is a ~120 kDa protein. Endosomal receptor that plays a key role in innate and adaptive immunity. Controls host immune response against pathogens through recognition of uridine-containing single strand RNAs (ssRNAs) of viral origin or guanosine analogs. Upon binding to agonists, undergoes dimerization that brings TIR domains from the two molecules into direct contact, leading to the recruitment of TIR-containing downstream adapter MYD88 through homotypic interaction. In turn, the Myddosome signaling complex is formed involving IRAK4, IRAK1, TRAF6, TRAF3 leading to activation of downstream transcription factors NF-kappa-B and IRF7 to induce pro-inflammatory cytokines and interferons, respectively. In plasmacytoid dendritic cells, RNASET2 endonuclease cooperates with PLD3 or PLD4 5'->3' exonucleases to process RNA and release 2',3'-cyclic guanosine monophosphate (2',3'-cGMP) and cytidine-rich RNA fragments that occupy TLR7 ligand-binding pockets and trigger a signaling-competent state.
References:
1. Diebold, SS. et al. (2004) Science (New York, N.Y.) 303, 1529-31. PMID: 14976261
2. Heinz, LX. et al. (2020) Nature 581, 316-322. PMID: 32433612
3. Lee, J. et al. (2003) Proceedings of the National Academy of Sciences of the United States of America 100, 6646-51. PMID: 12738885
4. Zhang, Z. et al. (2016) Immunity 45, 737-748. PMID: 27742543
5. Davenne, T. et al. (2020) European journal of immunology 50, 56-62. PMID: 31608988
6. van der Made, CI. et al. (2020) JAMA 324, 663-673. PMID: 32706371
7. Brown, GJ. et al. (2022) Nature 605, 349-356. PMID: 35477763
Note: For research use only.