Anti-Human APLN/Apelin Antibody (SAA1754)

Description: Anti-Human APLN/Apelin Antibody (SAA1754) [SAA1754] (RA12398) is a mouse monoclonal antibody detecting Apelin in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: APEL, Apelin, APJ endogenous ligand, APLN
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Apelin (APLN) is a ~8 kDa protein. Peptide hormone that functions as endogenous ligand for the G-protein-coupled apelin receptor (APLNR/APJ), that plays a role in cadiovascular homeostasis. Functions as a balanced agonist activating both G(i) protein pathway and beta-arrestin pathway of APLNR. Downstream G proteins activation, apelin can inhibit cAMP production and activate key intracellular effectors such as ERKs. On the other hand, APLNR activation induces beta-arrestin recruitment to the membrane leading to desensitization and internalization of the receptor. Apelin blunts cardiac hypertrophic induction from APLNR on response to pathological stimuli, but also induces myocardial hypertrophy under normal conditions.
References:
1. Habata, Y. et al. (1999) Biochimica et biophysica acta 1452, 25-35. PMID: 10525157
2. Scimia, MC. et al. (2012) Nature 488, 394-8. PMID: 22810587
3. Yue, Y. et al. (2022) Nature structural & molecular biology 29, 688-697. PMID: 35817871
4. Wang, WW. et al. (2024) Cell 187, 1460-1475.e20. PMID: 38428423
Note: For research use only.