Anti-Human EMC10 Antibody (SAA1827)

Description: Anti-Human EMC10 Antibody (SAA1827) [SAA1827] (RA12471) is a mouse monoclonal antibody detecting EMC10 in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: Hematopoietic signal peptide-containing membrane domain-containing protein 1, EMC10, INM02, C19orf63, ER membrane protein complex subunit 10
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: ER membrane protein complex subunit 10 (EMC10) is a ~27 kDa protein. Part of the endoplasmic reticulum membrane protein complex (EMC) that enables the energy-independent insertion into endoplasmic reticulum membranes of newly synthesized membrane proteins. Preferentially accommodates proteins with transmembrane domains that are weakly hydrophobic or contain destabilizing features such as charged and aromatic residues. Involved in the cotranslational insertion of multi-pass membrane proteins in which stop-transfer membrane-anchor sequences become ER membrane spanning helices. It is also required for the post-translational insertion of tail-anchored/TA proteins in endoplasmic reticulum membranes. By mediating the proper cotranslational insertion of N-terminal transmembrane domains in an N-exo topology, with translocated N-terminus in the lumen of the ER, controls the topology of multi-pass membrane proteins like the G protein-coupled receptors.
References:
1. Guna, A. et al. (2018) Science (New York, N.Y.) 359, 470-473. PMID: 29242231
2. Shurtleff, MJ. et al. (2018) eLife 7. PMID: 29809151
3. Chitwood, PJ. et al. (2018) Cell 175, 1507-1519.e16. PMID: 30415835
4. Pleiner, T. et al. (2020) Science (New York, N.Y.) 369, 433-436. PMID: 32439656
5. O'Donnell, JP. et al. (2020) eLife 9. PMID: 32459176
6. Reboll, MR. et al. (2017) Circulation 136, 1809-1823. PMID: 28931551
Note: For research use only.