Anti-Human RSPO2 Antibody (SAA1853)

Description: Anti-Human RSPO2 Antibody (SAA1853) [SAA1853] (RA12497) is a mouse monoclonal antibody detecting RSPO2 in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: Roof plate-specific spondin-2, R-spondin-2, hRspo2, RSPO2
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: R-spondin-2 (RSPO2) is a ~28 kDa protein. Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. During embryonic development, plays a crucial role in limb specification, amplifying the Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3, hence governing the number of limbs an embryo should form.
References:
1. de Lau, W. et al. (2011) Nature 476, 293-7. PMID: 21727895
2. Glinka, A. et al. (2011) EMBO reports 12, 1055-61. PMID: 21909076
3. Gong, X. et al. (2012) PloS one 7, e37137. PMID: 2261592
4. Szenker-Ravi, E. et al. (2018) Nature 557, 564-569. PMID: 29769720
Note: For research use only.