Anti-Human DDX4/Vasa Antibody (SAA1970)

Description: Anti-Human DDX4/Vasa Antibody (SAA1970) [SAA1970] (RA12614) is a mouse monoclonal antibody detecting DDX4 in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: DDX4, VASA, Vasa homolog, DEAD box protein 4, Probable ATP-dependent RNA helicase DDX4
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Probable ATP-dependent RNA helicase DDX4 (Vasa) is a ~79 kDa protein. ATP-dependent RNA helicase required during spermatogenesis. Required to repress transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposons. Involved in the secondary piRNAs metabolic process, the production of piRNAs in fetal male germ cells through a ping-pong amplification cycle. Required for PIWIL2 slicing-triggered piRNA biogenesis: helicase activity enables utilization of one of the slice cleavage fragments generated by PIWIL2 and processing these pre-piRNAs into piRNAs.
References:
1. Castrillon, DH. et al. (2000) Proceedings of the National Academy of Sciences of the United States of America 97, 9585-90. PMID: 10920202
2. Li, HJ. et al. (2010) Chinese medical journal 123, 2879-82. PMID: 21034600
Note: For research use only.