Anti-Human HLA-G Antibody (38358#)

Description: Anti-Human HLA-G Antibody (38358#) [38358#] (RA12759) is a mouse monoclonal antibody detecting HLA-6.0 in FCM. Suitable for Human.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications.
Host species: Mouse
Isotype: IgG1
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: FCM
Target: HLAG, HLA-G, sHLA-G, HLA G antigen, MHC class I antigen G, HLA-6.0, HLA class I histocompatibility antigen, alpha chain G
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: HLA class I histocompatibility antigen, alpha chain G (HLA-G) is a ~38 kDa protein. Non-classical major histocompatibility class Ib molecule involved in immune regulatory processes at the maternal-fetal interface. In complex with B2M/beta-2 microglobulin binds a limited repertoire of nonamer self-peptides derived from intracellular proteins including histones and ribosomal proteins. Peptide-bound HLA-G-B2M complex acts as a ligand for inhibitory/activating KIR2DL4, LILRB1 and LILRB2 receptors on uterine immune cells to promote fetal development while maintaining maternal-fetal tolerance. Upon interaction with KIR2DL4 and LILRB1 receptors on decidual NK cells, it triggers NK cell senescence-associated secretory phenotype as a molecular switch to promote vascular remodeling and fetal growth in early pregnancy. Through interaction with KIR2DL4 receptor on decidual macrophages induces pro-inflammatory cytokine production mainly associated with tissue remodeling.
References:
1. Li, C. et al. (2009) Proceedings of the National Academy of Sciences of the United States of America 106, 5767-72. PMID: 19304799
2. Rajagopalan, S. et al. (2012) Proceedings of the National Academy of Sciences of the United States of America 109, 20596-601. PMID: 23184984
3. Fu, B. et al. (2017) Immunity 47, 1100-1113.e6. PMID: 29262349
4. Lee, N. et al. (1995) Immunity 3, 591-600. PMID: 7584149
5. Diehl, M. et al. (1996) Current biology : CB 6, 305-14. PMID: 8805247
6. Rajagopalan, S. et al. (2006) PLoS biology 4, e9. PMID: 16366734
7. Gregori, S. et al. (2010) Blood 116, 935-44. PMID: 2044811
8. Köstlin, N. et al. (2017) European journal of immunology 47, 374-384. PMID: 27859042
9. Rajagopalan, S. et al. (1999) The Journal of experimental medicine 189, 1093-100. PMID: 10190900
Note: For research use only.