Anti-Sahara scorpion AaH II/Neurotoxin II Nanobody (SAA2039)

Description: Anti-Sahara scorpion AaH II/Neurotoxin II Nanobody (SAA2039) [SAA2039] (RA13858) is a alpaca monoclonal antibody detecting Neurotoxin II in ELISA, SPR.
Host species: Alpaca
Isotype: VHH-8His-Cys-tag
Species reactivity: Androctonus australis (Sahara scorpion)
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, SPR
Target: Alpha-mammal toxin AaH2, AaHII, Neurotoxin 2, Neurotoxin II
Purification: Purified by Nickel column.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Alpha-mammal toxin AaH2 (AaH II/Neurotoxin II) is a ~9 kDa protein. Alpha toxin that binds voltage-independently at site-3 of sodium channels (Nav), inhibits the inactivation of the activated channels, and weakly inhibits activation, thereby blocking neuronal transmission. Inserts into voltage-sensing domain IV to stabilize a deactivated state, thereby preventing fast-inactivation. Principally slows the inactivation process of TTX-sensitive sodium channels. It is active on mammalian brain Nav1.2/SCN2A (EC(50)human=0.72 nM, EC(50)rat=2.6 nM), on rat skeletal muscle Nav1.4/SCN4A (EC(50)=2.2 nM), and on human neuronal Nav1.7/SCN9A (EC(50)=6.8-51.7 nM). In vivo, intraplantar injection into mice induces spontaneous pain responses.
References:
1. Clairfeuille, T. et al. (2019) Science (New York, N.Y.) 363. PMID: 30733386
2. Abbas, N. et al. (2013) Pain 154, 1204-15. PMID: 23685008
3. Alami, M. et al. (2003) The Biochemical journal 375, 551-60. PMID: 12911331
Note: For research use only.