Anti-Clostridium perfringens pfo/Perfringolysin O/Theta-toxin Antibody (HS1)

Host species: Mouse
Isotype: IgG2a
Species reactivity: Clostridium perfringens (strain 13 / Type A), Streptococcus dysgalactiae subsp. equisimilis (Streptococcus equisimilis)
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1.06 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, Neutralization, WB
Target: Perfringolysin O, PFO, Theta-toxin, Thiol-activated cytolysin, pfo, pfoA, pfoR, CPE0163, Streptolysin O, SLO, Thiol-activated cytolysin, slo, Clostridium perfringens (strain 13 / Type A), Streptococcus dysgalactiae subsp. equisimilis (Streptococcus equisimilis)
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Perfringolysin O (pfo/Theta-toxin) is a ~55 kDa protein. A cholesterol-dependent toxin that causes cytolysis by forming pores in cholesterol-containing host membranes. After binding to target membranes, the protein assembles into a pre-pore complex. A major conformational change leads to insertion in the host membrane and formation of an oligomeric pore complex. Cholesterol is required for binding to host cell membranes, membrane insertion and pore formation; cholesterol binding is mediated by a Thr-Leu pair in the C-terminus. Can be reversibly inactivated by oxidation.
Note: For research use only.