Anti-South American/Mojave rattlesnake crotoxin Antibody (Al0G)

Description: Anti-South American/Mojave rattlesnake crotoxin Antibody (Al0G) [Al0G] (RA13883) is a mouse monoclonal antibody detecting Crotalus durissus terrificus (South American rattlesnake) in ELISA, FCM, IF, SPR, WB.
Highlights:
• Multi-Application — Validated across multiple applications.
• Flow Cytometry — Validated for flow cytometry applications.
Host species: Mouse
Isotype: IgG2a
Species reactivity: Crotalus durissus terrificus (South American rattlesnake), Crotalus scutulatus scutulatus (Mojave rattlesnake)
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, FCM, IF, SPR, WB
Target: Phospholipase A2 crotoxin basic subunit CBc, CB1, CTX subunit CBc, svPLA2, 3.1.1.4, Phosphatidylcholine 2-acylhydrolase, Basic phospholipase A2 Mtx-b, svPLA2, 3.1.1.4, Mojave toxin basic chain, Phosphatidylcholine 2-acylhydrolase, Phospholipase A2 CB1, Crotalus durissus terrificus (South American rattlesnake), Crotalus scutulatus scutulatus (Mojave rattlesnake)
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Phospholipase A2 crotoxin basic subunit CBc is a ~15 kDa protein. Heterodimer CA-CB: Crotoxin is a potent presynaptic neurotoxin that possesses phospholipase A2 (PLA2) activity and exerts a lethal action by blocking neuromuscular transmission. It consists of a non-covalent association of a basic and weakly toxic PLA2 subunit (CBa2, CBb, CBc, or CBd), with a small acidic, non-enzymatic and non-toxic subunit (CA1, CA2, CA3 or CA4). The complex acts by binding to a specific 48-kDa protein (R48) receptor located on presynaptic membranes, forming a transient ternary complex CA-CB-R48, followed by dissociation of the CA-CB complex and release of the CA subunit. At equilibrium, only the CB subunits remain associated with the specific crotoxin receptor. In addition to neurotoxicity, crotoxin has been found to exert myotoxicity, nephrotoxicity, and cardiovascular toxicity.
References:
1. Sampaio, SC. et al. (2010) Toxicon : official journal of the International Society on Toxinology 55, 1045-60. PMID: 20109480
Note: For research use only.