Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (QD20)

Description: Anti-Staphylococcus aureus Alpha-toxin/hly/AT Antibody (QD20) [QD20] (RA13892) is a mouse monoclonal antibody detecting Staphylococcus aureus in ELISA, Neutralization, SPR.
Highlights:
• Multi-Application — Validated across multiple applications.
Host species: Mouse
Isotype: IgG2a
Species reactivity: Staphylococcus aureus, Staphylococcus aureus (strain NCTC 8325 / PS 47)
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, Neutralization, SPR
Target: hly, hla, Alpha-hemolysin, Alpha-toxin, Alpha-HL, AT, Staphylococcus aureus, Staphylococcus aureus (strain NCTC 8325 / PS 47)
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Alpha-hemolysin (Alpha-toxin/hly) is a ~35 kDa protein. Alpha-toxin binds to the membrane of eukaryotic cells (particularly red blood cells, RBC) forming pores, resulting in hemolysis, with the release of low-molecular weight molecules leading to eventual osmotic RBC lysis. Human RBCs bind much less alpha-toxin than do rabbit RBCs. Heptamer oligomerization and pore formation is required for lytic activity.
References:
1. Walker, B. et al. (1992) The Journal of biological chemistry 267, 10902-9. PMID: 1587866
2. Wilke, GA. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 13473-8. PMID: 20624979
Note: For research use only.