Anti-Mouse CD5/Ly-1 Antibody (SAA2085)

Description: Anti-Mouse CD5/Ly-1 Antibody (SAA2085) [SAA2085] (RA14207) is a human monoclonal antibody detecting Ly-1 in ELISA. Suitable for Mouse.
Host species: Human
Isotype: VHH-hFc
Species reactivity: Mouse
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: Lyt-1, Cd5, CD5, T-cell surface glycoprotein CD5, Ly-1, Lymphocyte antigen 1
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: T-cell surface glycoprotein CD5 (Ly-1) is a ~53 kDa protein. Lymphoid-specific receptor expressed by all T-cells and in a subset of B-cells known as B1a cells. Plays a role in the regulation of TCR and BCR signaling, thymocyte selection, T-cell effector differentiation and immune tolerance. Acts by interacting with several ligands expressed on B-cells such as CD5L or CD72 and thereby plays an important role in contact-mediated, T-dependent B-cell activation and in the maintenance of regulatory T and B-cell homeostasis. Functions as a negative regulator of TCR signaling during thymocyte development by associating with several signaling proteins including LCK, CD3Z chain, PI3K or CBL. Mechanistically, co-engagement of CD3 with CD5 enhances phosphorylated CBL recruitment leading to increased VAV1 phosphorylation and degradation.
References:
1. Schuster, C. et al. (2022) Frontiers in immunology 13, 906499. PMID: 35720357
2. Zheng, M. et al. (2014) Immunological investigations 43, 705-16. PMID: 24950378
3. Biancone, L. et al. (1996) The Journal of experimental medicine 184, 811-9. PMID: 9064341
4. Bikah, G. et al. (1998) International immunology 10, 1185-96. PMID: 9723705
5. Dennehy, KM. et al. (2001) International immunology 13, 149-56. PMID: 11157848
Note: For research use only.