Anti-Human BAX Nanobody (SAA2133)

Description: Anti-Human BAX Nanobody (SAA2133) [SAA2133] (RA14352) is a alpaca monoclonal antibody detecting BAX in ELISA, SPR. Suitable for Human.
Host species: Alpaca
Isotype: VHH-8His-Cys-tag
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, SPR
Target: BAX, BCL2L4, Apoptosis regulator BAX, Bcl-2-like protein 4
Purification: Purified by Nickel column.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Apoptosis regulator BAX is a ~21 kDa protein. Plays a role in the mitochondrial apoptotic process. Under normal conditions, BAX is largely cytosolic via constant retrotranslocation from mitochondria to the cytosol mediated by BCL2L1/Bcl-xL, which avoids accumulation of toxic BAX levels at the mitochondrial outer membrane (MOM). Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis.
References:
1. Schmitt, E. et al. (2000) Biochemical and biophysical research communications 270, 868-79. PMID: 10772918
2. Tan, KO. et al. (2001) The Journal of biological chemistry 276, 2802-7. PMID: 11060313
3. Zhang, H. et al. (2005) Nature cell biology 7, 909-15. PMID: 16113678
4. Tan, KO. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 14623-8. PMID: 16199525
5. Gavathiotis, E. et al. (2008) Nature 455, 1076-81. PMID: 18948948
6. Czabotar, PE. et al. (2011) The Journal of biological chemistry 286, 7123-31. PMID: 21199865
7. Edlich, F. et al. (2011) Cell 145, 104-16. PMID: 2145867
8. Wei, B. et al. (2015) Journal of virology 89, 3804-18. PMID: 25609812
9. Choi, HS. et al. (2022) International journal of molecular sciences 23. PMID: 36361894
10. Oltvai, ZN. et al. (1993) Cell 74, 609-19. PMID: 8358790
Note: For research use only.