Anti-Human CD31/PECAM1 Antibody (SAA2178)

Description: Anti-Human CD31/PECAM1 Antibody (SAA2178) [SAA2178] (RA14404) is a mouse monoclonal antibody detecting PECAM1 in ELISA, WB, FCM. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
• Flow Cytometry — Validated for flow cytometry applications.
Host species: Mouse
Isotype: VHH-mFc (IgG2a)
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, WB
Target: Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Platelet endothelial cell adhesion molecule (CD31/PECAM1) is a ~82 kDa protein. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal.
References:
1. Sachs, UJ. et al. (2007) The Journal of biological chemistry 282, 23603-12. PMID: 17580308
2. Dasgupta, B. et al. (2009) Journal of immunology (Baltimore, Md. : 1950) 182, 5041-51. PMID: 19342684
3. Hu, M. et al. (2016) Scientific reports 6, 38655. PMID: 27958302
4. Brown, S. et al. (2002) Nature 418, 200-3. PMID: 12110892
5. Yeh, JC. et al. (2008) Biochemistry 47, 9029-39. PMID: 18672896
Note: For research use only.