Anti-Human CASP3 Nanobody (VHH1)

Description: Anti-Human CASP3 Nanobody (VHH1) [VHH1] (RA14456) is a alpaca monoclonal antibody detecting CASP3 in ELISA. Suitable for Human.
Host species: Alpaca
Isotype: VHH-8His-Cys-tag
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: Caspase-3, CPP-32, CPP32, CASP-3, SREBP cleavage activity 1, CASP3, Apopain, SCA-1, Cysteine protease CPP32, Protein Yama
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Caspase-3 (CASP3) is a ~31 kDa protein. Thiol protease that acts as a major effector caspase involved in the execution phase of apoptosis. Following cleavage and activation by initiator caspases (CASP8, CASP9 and/or CASP10), mediates execution of apoptosis by catalyzing cleavage of many proteins. At the onset of apoptosis, it proteolytically cleaves poly(ADP-ribose) polymerase PARP1 at a '216-Asp-|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9 (CASP6, CASP7 and CASP9, respectively).
References:
1. Walsh, JG. et al. (2008) Proceedings of the National Academy of Sciences of the United States of America 105, 12815-9. PMID: 1872368
2. Nakatsumi, H. et al. (2010) The Journal of biological chemistry 285, 25418-25. PMID: 2056663
3. Thomsen, ND. et al. (2013) Proceedings of the National Academy of Sciences of the United States of America 110, 8477-82. PMID: 23650375
4. Peng, T. et al. (2022) Molecular cell 82, 1806-1820.e8. PMID: 35338844
5. Liu, Y. et al. (2022) mBio 13, e0069022. PMID: 3544612
6. Nicholson, DW. et al. (1995) Nature 376, 37-43. PMID: 75964301
7. Shi, X. et al. (2023) Nature 624, 442-450. PMID: 37993714
Note: For research use only.