Anti-Human CXCL4/PF4 Antibody (1E12#)

Description: Anti-Human CXCL4/PF4 Antibody (1E12#) [1E12#] (RA14578) is a mouse monoclonal antibody detecting CXCL4 in ELISA. Suitable for Human.
Host species: Mouse
Isotype: IgG2a, kappa
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA
Target: C-X-C motif chemokine 4, PF-4, Oncostatin-A, Endothelial cell growth inhibitor, CXCL4, Iroplact, Platelet factor 4, PF4, SCYB4
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Platelet factor 4 (CXCL4/PF4) is a ~10 kDa protein. Chemokine released during platelet aggregation that plays a role in different biological processes including hematopoiesis, cell proliferation, differentiation, and activation. Acts via different functional receptors including CCR1, CXCR3A or CXCR3B. Upon interaction with CXCR3A receptor, induces activated T-lymphocytes migration mediated via downstream Ras/extracellular signal-regulated kinase (ERK) signaling. Neutralizes the anticoagulant effect of heparin by binding more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Plays a role in the inhibition of hematopoiesis and in the maintenance of hematopoietic stem cell (HSC) quiescence.
References:
1. Fox, JM. et al. (2018) Scientific reports 8, 9466. PMID: 29930254
2. Lecomte-Raclet, L. et al. (1998) Blood 91, 2772-80. PMID: 9531587
3. Mueller, A. et al. (2008) Journal of leukocyte biology 83, 875-82. PMID: 18174362
4. Van Raemdonck, K. et al. (2014) Angiogenesis 17, 631-40. PMID: 24469069
5. Yang, C. et al. (2022) Nature communications 13, 3426. PMID: 35701499
6. Affandi, AJ. et al. (2022) Cell reports 38, 110189. PMID: 34986347
7. Dudek, AZ. et al. (1997) The Journal of biological chemistry 272, 31785-92. PMID: 9395524
Note: For research use only.