Anti-Human MBTD1 IP Antibody (SAA0210)

Description: Anti-Human MBTD1 IP Antibody (SAA0210) [SAA0210] (RA14605) is a mouse monoclonal antibody detecting MBTD1 in ELISA, IF, IP, IP-MS. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
Host species: Mouse
Isotype: IgG
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, IF, IP, IP-MS
Target: MBTD1, MBT domain-containing protein 1
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: MBT domain-containing protein 1 (MBTD1) is a ~70 kDa protein. Chromatin reader component of the NuA4 histone acetyltransferase complex, a multiprotein complex involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. The NuA4 complex plays a direct role in repair of DNA double-strand breaks (DSBs) by promoting homologous recombination (HR). MBTD1 specifically recognizes and binds monomethylated and dimethylated 'Lys-20' on histone H4 (H4K20me1 and H4K20me2, respectively). In the NuA4 complex, MBTD1 promotes recruitment of the complex to H4K20me marks by competing with TP53BP1 for binding to H4K20me. Following recruitment to H4K20me at DNA breaks, the NuA4 complex catalyzes acetylation of 'Lys-15' on histone H2A (H2AK15), blocking the ubiquitination mark required for TP53BP1 localization at DNA breaks, thereby promoting homologous recombination (HR).
References:
1. Jacquet, K. et al. (2016) Molecular cell 62, 409-421. PMID: 27153538
2. Zhang, H. et al. (2020) Cell reports 30, 3996-4002.e4. PMID: 32209463
3. Eryilmaz, J. et al. (2009) PloS one 4, e7274. PMID: 19841675
Note: For research use only.