Anti-Human PHF8 IP Antibody (SAA0216)

Description: Anti-Human PHF8 IP Antibody (SAA0216) [SAA0216] (RA14611) is a mouse monoclonal antibody detecting PHF8 in ELISA, IF, IP. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
Host species: Mouse
Isotype: IgG2a
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, IF, IP
Target: [histone H3]-dimethyl-L-lysine(9) demethylase PHF8, KIAA1111, [histone H3]-dimethyl-L-lysine(36) demethylase PHF8, PHF8, ZNF422, Histone lysine demethylase PHF8, PHD finger protein 8
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Histone lysine demethylase PHF8 is a ~117 kDa protein. Histone lysine demethylase with selectivity for mono- and dimethylated residues. It plays an essential role in cell cycle progression and rDNA transcription. Demethylates mono- and dimethylated histone H3 'Lys-9' residue (H3K9Me1 and H3K9Me2) and monomethylated histone H4 'Lys-20' residue (H4K20Me1). Acts as a transcription activator as H3K9Me1, H3K9Me2, H3K27Me2 and H4K20Me1 are epigenetic repressive marks. Displays a very low intrinsic activity toward dimethylated H3 'Lys-27' (H3K27Me2).
References:
1. Loenarz, C. et al. (2010) Human molecular genetics 19, 217-22. PMID: 19843542
2. Zhu, Z. et al. (2010) Cell research 20, 794-801. PMID: 20531378
3. Qiu, J. et al. (2010) Cell research 20, 908-18. PMID: 20548336
4. Liu, W. et al. (2010) Nature 466, 508-12. PMID: 20622854
5. Yu, L. et al. (2010) Cell research 20, 166-73. PMID: 20101266
6. Feng, W. et al. (2010) Nature structural & molecular biology 17, 445-50. PMID: 20208542
7. Kleine-Kohlbrecher, D. et al. (2010) Molecular cell 38, 165-78. PMID: 2034672
8. Qi, HH. et al. (2010) Nature 466, 503-7. PMID: 20622853
9. Horton, JR. et al. (2010) Nature structural & molecular biology 17, 38-43. PMID: 20023638
Note: For research use only.