Anti-Human SETMAR IP Antibody (SAA0224)

Description: Anti-Human SETMAR IP Antibody (SAA0224) [SAA0224] (RA14619) is a mouse monoclonal antibody detecting SETMAR in ELISA, IF, IP. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
Host species: Mouse
Isotype: IgG2a
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, IF, IP
Target: Histone-lysine N-methyltransferase SETMAR, SETMAR, SET domain and mariner transposase fusion protein, Metnase
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Histone-lysine N-methyltransferase SETMAR is a ~78 kDa protein. Protein derived from the fusion of a methylase with the transposase of an Hsmar1 transposon that plays a role in DNA double-strand break repair, stalled replication fork restart and DNA integration. DNA-binding protein, it is indirectly recruited to sites of DNA damage through protein-protein interactions. Also has kept a sequence-specific DNA-binding activity recognizing the 19-mer core of the 5'-terminal inverted repeats (TIRs) of the Hsmar1 element and displays a DNA nicking and end joining activity. In parallel, has a histone methyltransferase activity and methylates 'Lys-4' and 'Lys-36' of histone H3. Specifically mediates dimethylation of H3 'Lys-36' at sites of DNA double-strand break and may recruit proteins required for efficient DSB repair through non-homologous end-joining.
References:
1. Lee, SH. et al. (2005) Proceedings of the National Academy of Sciences of the United States of America 102, 18075-80. PMID: 16332963
2. Cordaux, R. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 8101-6. PMID: 16672366
3. Miskey, C. et al. (2007) Molecular and cellular biology 27, 4589-600. PMID: 17403897
4. Roman, Y. et al. (2007) Biochemistry 46, 11369-76. PMID: 17877369
5. Beck, BD. et al. (2008) The Journal of biological chemistry 283, 9023-30. PMID: 18263876
6. Goodwin, KD. et al. (2010) Biochemistry 49, 5705-13. PMID: 20521842
7. Hromas, R. et al. (2012) Oncogene 31, 4245-54. PMID: 22231448
8. Kim, HS. et al. (2014) The Journal of biological chemistry 289, 10930-10938. PMID: 24573677
9. Fnu, S. et al. (2011) Proceedings of the National Academy of Sciences of the United States of America 108, 540-5. PMID: 21187428
10. Williamson, EA. et al. (2008) Nucleic acids research 36, 5822-31. PMID: 18790802
Note: For research use only.