Anti-Human DNMT1 IP Antibody (SAA0251)

Description: Anti-Human DNMT1 IP Antibody (SAA0251) [SAA0251] (RA14646) is a mouse monoclonal antibody detecting DNMT1 in ELISA, IF, IP. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
Host species: Mouse
Isotype: IgG2a
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, IF, IP
Target: CXXC-type zinc finger protein 9, MCMT, AIM, DNMT1, DNA MTase HsaI, DNMT, DNA (cytosine-5)-methyltransferase 1, Dnmt1, DNA methyltransferase HsaI, M.HsaI, CXXC9
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: DNA (cytosine-5)-methyltransferase 1 (DNMT1) is a ~183 kDa protein. DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development.
References:
1. Schlesinger, Y. et al. (2007) Nature genetics 39, 232-6. PMID: 1720067
2. Pradhan, M. et al. (2008) Biochemistry 47, 10000-9. PMID: 18754681
3. Felle, M. et al. (2011) Nucleic acids research 39, 8355-65. PMID: 21745816
4. Zhang, ZM. et al. (2015) Journal of molecular biology 427, 2520-2531. PMID: 26070743
5. Viré, E. et al. (2006) Nature 439, 871-4. PMID: 16357870
6. Serra, RW. et al. (2014) eLife 3, e02313. PMID: 24623306
Note: For research use only.