Anti-Human CBX3 IP Antibody (SAA0259)

Description: Anti-Human CBX3 IP Antibody (SAA0259) [SAA0259] (RA14654) is a mouse monoclonal antibody detecting CBX3 in ELISA, IF, IP. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
Host species: Mouse
Isotype: IgG1
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, IF, IP
Target: HECH, Heterochromatin protein 1 homolog gamma, Modifier 2 protein, Chromobox protein homolog 3, HP1 gamma, CBX3
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names:
Background: Chromobox protein homolog 3 (CBX3) is a ~20 kDa protein. Component of heterochromatin, which recognizes and binds histone H3 tails methylated at 'Lys-9', leading to epigenetic repression. Also recognizes and binds histone H1.4 methylated at 'Lys-26' (H1.4K26me). Excluded from chromatin when histone H1.4 is Simultaneously methylated at Lys-26 (H1.4K26me) and phosphorylated at Ser-27 (H1.4S27Ph). Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. Contributes to the conversion of local chromatin to a heterochromatin-like repressive state through H3 'Lys-9' trimethylation, mediates the recruitment of the methyltransferases SUV39H1 and/or SUV39H2 by the PER complex to the E-box elements of the circadian target genes such as PER2 itself or PER1.
References:
1. Lachner, M. et al. (2001) Nature 410, 116-20. PMID: 11242053
2. Daujat, S. et al. (2005) The Journal of biological chemistry 280, 38090-5. PMID: 16127177
3. Obuse, C. et al. (2004) Nature cell biology 6, 1135-41. PMID: 15502821
4. Bot, C. et al. (2017) Journal of cell science 130, 1134-1146. PMID: 28167679
Note: For research use only.