Anti-Human TSHR/LGR3 Antibody (K1-70)
Hot- Catalog number: FC10024
- Availability: In Stock
$191.00
- Ex Tax: $191.00
- Description: Anti-Human TSHR/LGR3 Antibody (K1-70) [K1-70] (FC10024) is a mouse monoclonal antibody detecting TSHR in Blocking, ELISA, FCM, WB. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
• Flow Cytometry — Validated for flow cytometry applications. - Model: HB852207
- Name: Anti-Human TSHR/LGR3 Antibody (K1-70)
- Host species: Mouse
- Isotype: IgG2a, lambda
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4.
- Concentration: 0.56 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: Blocking, ELISA, FCM, WB
- Target: Thyroid-stimulating hormone receptor, TSH-R, TSHR, Thyrotropin receptor, LGR3, RHD18102
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: Thyrotropin receptor (TSHR/LGR3) is a ~86 kDa protein. Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. TSHR is coupled to G(s) proteins and mediates the activation of adenylate cyclase. This leads to the generation of cyclic adenosine monophosphate (cAMP), which in turn activates protein kinase A (PKA). PKA subsequently phosphorylates downstream targets involved in thyroid hormone biosynthesis and secretion, including thyroid peroxidase (TPO) and the sodium/iodide symporter (NIS).
References:
1. Costagliola, S. et al. (2002) The EMBO journal 21, 504-13. PMID: 11847099
2. Nakabayashi, K. et al. (2002) The Journal of clinical investigation 109, 1445-52. PMID: 12045258
3. Faust, B. et al. (2022) Nature 609, 846-853. PMID: 35940205
4. Duan, J. et al. (2022) Nature 609, 854-859. PMID: 35940204
5. Morgan, SJ. et al. (2016) Thyroid : official journal of the American Thyroid Association 26, 1794-1803. PMID: 27638195 - Note: For research use only.
Images
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Flow CytometryFlow-cytometry using anti-human TSHR antibody.TSHR Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human TSHR antibody monoclonal antibody (Catalog # HB852207 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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Western blotWestern blot analysis was performed using anti-TSHR monoclonal antibody at 1ug/mL on various samples.
Lane 1: Mouse ovary lysate
