Anti-Human TRGV9/TCR Vγ 9 Antibody (7A5)
- Catalog number: FC10026
- Availability: In Stock
$191.00
- Ex Tax: $191.00
- Description: Anti-Human TRGV9/TCR Vγ 9 Antibody (7A5) [7A5] (FC10026) is a mouse monoclonal antibody detecting TRGV9 in FCM. Suitable for Human.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications. - Model: HP264107
- Name: Anti-Human TRGV9/TCR Vγ 9 Antibody (7A5)
- Host species: Mouse
- Isotype: IgG1, kappa
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4.
- Concentration: 1.79 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: FCM
- Target: T cell receptor gamma variable 9, TRGV9, TCRGV9
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: T cell receptor gamma variable 9 (TRGV9/Vγ 9) is a ~13 kDa protein. V region of the variable domain of T cell receptor (TR) gamma chain that participates in the antigen recognition. Gamma-delta TRs recognize a variety of self and foreign non-peptide antigens frequently expressed at the epithelial boundaries between the host and external environment, including endogenous lipids presented by MH-like protein CD1D and phosphoantigens presented by butyrophilin-like molecule BTN3A1. Upon antigen recognition induces rapid, innate-like immune responses involved in pathogen clearance and tissue repair. Binding of gamma-delta TR complex to antigen triggers phosphorylation of immunoreceptor tyrosine-based activation motifs (ITAMs) in the CD3 chains by the LCK and FYN kinases, allowing the recruitment, phosphorylation, and activation of ZAP70 that facilitates phosphorylation of the scaffolding proteins LCP2 and LAT. This lead to the formation of a supramolecular signalosome that recruits the phospholipase PLCG1, resulting in calcium mobilization and ERK activation, ultimately leading to T cell expansion and differentiation into effector cells.
References:
1. Lefranc, MP. (2014) Frontiers in immunology 5, 22. PMID: 24600447
2. Vantourout, P. et al. (2013) Nature reviews. Immunology 13, 88-100. PMID: 23348415
3. Nielsen, MM. et al. (2017) Nature reviews. Immunology 17, 733-745. PMID: 28920588
4. Ribeiro, ST. et al. (2015) Frontiers in immunology 6, 15. PMID: 25674089
5. Chien, YH. et al. (2014) Annual review of immunology 32, 121-55. PMID: 24387714 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Anti-Human TRGV9/TCR Vγ?9 Antibody (7A5)
