Anti-Human CD14 Antibody (F1031)
- Catalog number: FC10159
- Availability: In Stock
$196.00
- Ex Tax: $196.00
- Description: Anti-Human CD14 Antibody (F1031) [F1031] (FC10159) is a human monoclonal antibody detecting CD14 in ELISA, FCM, IHC, WB. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
• Flow Cytometry — Validated for flow cytometry applications. - Model: HY038307
- Name: Anti-Human CD14 Antibody (F1031)
- Host species: Human
- Isotype: IgG1
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4.
- Concentration: 1 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: ELISA, FCM, IHC, WB
- Target: Myeloid cell-specific leucine-rich glycoprotein, CD14, Monocyte differentiation antigen CD14, RHC33702
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: Monocyte differentiation antigen CD14 is a ~40 kDa protein. Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL(-)) and mediates the cytokine release induced by LDL(-).
References:
1. Wright, SD. et al. (1990) Science (New York, N.Y.) 249, 1431-3. PMID: 1698311
2. Kelley, SL. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 190, 1304-11. PMID: 23264655
3. Tsukamoto, H. et al. (2010) International immunology 22, 271-80. PMID: 20133493
4. Murakami, K. et al. (2012) FEBS letters 586, 319-24. PMID: 22265692
5. Haziot, A. et al. (1996) Immunity 4, 407-14. PMID: 8612135
6. Triantafilou, M. et al. (2006) The Journal of biological chemistry 281, 31002-11. PMID: 16880211
7. Estruch, M. et al. (2013) Atherosclerosis 229, 356-62. PMID: 23880187 - Note: For research use only.
Images
-
SDS-PAGESDS-PAGE for Anti-Human CD14 Antibody (F1031)
Datasheet
