Anti-Human CD317/BST2 Antibody (HM1.24)
- Catalog number: FC10314
- Availability: In Stock
$191.00
- Ex Tax: $191.00
- Description: Anti-Human CD317/BST2 Antibody (HM1.24) [HM1.24] (FC10314) is a mouse monoclonal antibody detecting CD317 in FCM. Suitable for Human.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications. - Model: HC341107
- Name: Anti-Human CD317/BST2 Antibody (HM1.24)
- Host species: Mouse
- Isotype: IgG1, kappa
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4.
- Concentration: 1 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: FCM
- Target: Tetherin, Bone marrow stromal antigen 2, BST2, BST-2, CD317, HM1.24 antigen
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: Bone marrow stromal antigen 2 (CD317/BST2) is a ~19 kDa protein. IFN-induced antiviral host restriction factor which efficiently blocks the release of diverse mammalian enveloped viruses by directly tethering nascent virions to the membranes of infected cells. Acts as a direct physical tether, holding virions to the cell membrane and linking virions to each other. The tethered virions can be internalized by endocytosis and subsequently degraded or they can remain on the cell surface. In either case, their spread as cell-free virions is restricted. Its target viruses belong to diverse families, including retroviridae: human immunodeficiency virus type 1 (HIV-1), human immunodeficiency virus type 2 (HIV-2), simian immunodeficiency viruses (SIVs), equine infectious anemia virus (EIAV), feline immunodeficiency virus (FIV), prototype foamy virus (PFV), Mason-Pfizer monkey virus (MPMV), human T-cell leukemia virus type 1 (HTLV-1), Rous sarcoma virus (RSV) and murine leukemia virus (MLV), flavivirideae: hepatitis C virus (HCV), filoviridae: ebola virus (EBOV) and marburg virus (MARV), arenaviridae: lassa virus (LASV) and machupo virus (MACV), herpesviridae: kaposis sarcoma-associated herpesvirus (KSHV), rhabdoviridae: vesicular stomatitis virus (VSV), orthomyxoviridae: influenza A virus, paramyxoviridae: nipah virus, and coronaviridae: SARS-CoV.
References:
1. Neil, SJ. et al. (2008) Nature 451, 425-30. PMID: 18200009
2. Van Damme, N. et al. (2008) Cell host & microbe 3, 245-52. PMID: 18342597
3. Jouvenet, N. et al. (2009) Journal of virology 83, 1837-44. PMID: 19036818
4. Perez-Caballero, D. et al. (2009) Cell 139, 499-511. PMID: 19879838
5. Miyakawa, K. et al. (2009) PLoS pathogens 5, e1000700. PMID: 20019814
6. Hinz, A. et al. (2010) Cell host & microbe 7, 314-323. PMID: 20399176
7. Pardieu, C. et al. (2010) PLoS pathogens 6, e1000843. PMID: 20419159
8. Yang, H. et al. (2010) Proceedings of the National Academy of Sciences of the United States of America 107, 18428-32. PMID: 2094032
9. Xu, F. et al. (2011) Virology journal 8, 198. PMID: 21529378
10. Dafa-Berger, A. et al. (2012) Virology 428, 98-111. PMID: 22520941 - Note: For research use only.
Images
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Flow CytometryFlow-cytometry using anti-human CD317 antibody.Jurkat cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD317 antibody monoclonal antibody (Catalog # HC341107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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Flow CytometryFlow-cytometry using anti-human CD317 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD317 antibody monoclonal antibody (Catalog # HC341107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
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Western blotWestern blot analysis was performed using anti-CD317 monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD317 (Catalog No: PH13671)
Lane 2: negative control
