Anti-Human CD37/TSPAN26 Antibody (SAA0012)
- Catalog number: FC10357
- Availability: In Stock
$196.00
- Ex Tax: $196.00
- Description: Anti-Human CD37/TSPAN26 Antibody (SAA0012) [SAA0012] (FC10357) is a human monoclonal antibody detecting TSPAN26 in FCM. Suitable for Human.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications. - Model: HY564207
- Name: Anti-Human CD37/TSPAN26 Antibody (SAA0012)
- Host species: Human
- Isotype: IgG1, kappa
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4.
- Concentration: 1 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: FCM
- Target: CD37, Tspan-26, TSPAN26, Leukocyte antigen CD37, Tetraspanin-26
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: Leukocyte antigen CD37 (TSPAN26) is a ~31 kDa protein. Structural component of specialized membrane microdomains known as tetraspanin-enriched microdomains (TERMs), which act as platforms for receptor clustering and signaling. Participates thereby in diverse biological functions such as cell signal transduction, adhesion, migration and protein trafficking. Upon ligand binding, two signaling pathways are activated, one acting through phosphorylation by LYN leading to cell death or a survival pathway with activation of GSK3B. Plays an essential role essential for clustering of integrin ITGA4/ITGB1 and promotes its mobility in the plasma membrane of B-cells. In turn, participates in ITGA4/ITGB1 integrin-mediated antiapoptotic signaling through AKT.
References:
1. Lapalombella, R. et al. (2012) Cancer cell 21, 694-708. PMID: 22624718
2. de Winde, CM. et al. (2016) The Journal of clinical investigation 126, 653-66. PMID: 26784544
3. Meyer-Wentrup, F. et al. (2007) Journal of immunology (Baltimore, Md. : 1950) 178, 154-62. PMID: 17182550 - Note: For research use only.
Images
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Flow CytometryFlow-cytometry using anti-human CD37 antibody. Human RPMI-8226 cell line were stained with an irrelevant antibody (Green Histogram) or an anti-human CD37 antibody monoclonal antibody (Catalog # HY564207 , Red Histogram) at a concentration of 20 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.n
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Western blotWestern blot analysis was performed using anti-IL23A monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human IL23A (Catalog No: PH12906)
Lane 2: negative control
