Anti-Human CD42b/GP1BA Antibody (5G6)

Size

50 µg

100 µg (+$107.00)

Qty

Description: Anti-Human CD42b/GP1BA Antibody (5G6) [5G6] (FC10374) is a mouse monoclonal antibody detecting CD42b in FCM, WB. Suitable for Human.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications.
Model: HY477107
Name: Anti-Human CD42b/GP1BA Antibody (5G6)
Host species: Mouse
Isotype: IgG1, kappa
Conjugation: Unconjugated
Species reactivity: Human
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1.48 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: FCM, WB
Target: CD42b, GP-Ib alpha, Antigen CD42b-alpha, GPIb-alpha, GPIbA, Glycoprotein Ibalpha, Platelet glycoprotein Ib alpha chain, GP1BA
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background: Platelet glycoprotein Ib alpha chain (CD42b/GP1BA) is a ~71 kDa protein. GP-Ib, a surface membrane protein of platelets, participates in the formation of platelet plugs by binding to the A1 domain of vWF, which is already bound to the subendothelium.
Note: For research use only.

Flow Cytometry

Flow-cytometry using anti-human CD42 antibody.Human peripheral blood monocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD42 antibody monoclonal antibody (Catalog # HY477107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CD42b/GP1BA monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human CD42b/GP1BA (Catalog No: PH15354)
Lane 2: negative control

Datasheet

$163.00