Anti-Human CD71/TFRC Antibody (JR-141)
- Catalog number: FC10425
- Availability: In Stock
$196.00
- Ex Tax: $196.00
- Description: Anti-Human CD71/TFRC Antibody (JR-141) [JR-141] (FC10425) is a human monoclonal antibody detecting CD71 in ELISA, FCM. Suitable for Human.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications. - Model: HY036107
- Name: Anti-Human CD71/TFRC Antibody (JR-141)
- Host species: Human
- Isotype: IgG1, kappa
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4.
- Concentration: 1.21 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: ELISA, FCM
- Target: Transferrin receptor protein 1, TfR1, T9, Trfr, p90, CD71, TFRC, TfR, sTfR, TR
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: Transferrin receptor protein 1 (CD71/TFRC) is a ~84 kDa protein. Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. A second ligand, the hereditary hemochromatosis protein HFE, competes for binding with transferrin for an overlapping C-terminal binding site.
References:
1. Senyilmaz, D. et al. (2015) Nature 525, 124-8. PMID: 26214738
2. Jabara, HH. et al. (2016) Nature genetics 48, 74-8. PMID: 26642240 - Note: For research use only.
Images
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BioactivitySEC-HPLC detection for Anti-Human CD71/TFRC Antibody (JR-141).
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Flow CytoMetryFlow-cytometry using anti-human CD71 antibody. U937 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human CD71 monoclonal antibody (Catalog: HY036107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
