Anti-Mouse CD152/CTLA4 Antibody (H11)

Size

50 µg

100 µg (+$179.00)

Qty

Description: Anti-Mouse CD152/CTLA4 Antibody (H11) [H11] (FC10608) is a mouse monoclonal antibody detecting CD152 in ELISA, FCM. Suitable for Mouse.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications.
Model: MB651107
Name: Anti-Mouse CD152/CTLA4 Antibody (H11)
Host species: Mouse
Isotype: VHH, mFc
Conjugation: Unconjugated
Species reactivity: Mouse
Form: Liquid
Storage buffer: 0.01M PBS, pH 7.4.
Concentration: 1 mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: ELISA, FCM
Target: Cytotoxic T-lymphocyte protein 4, Cytotoxic T-lymphocyte-associated antigen 4, CTLA-4, CD152, Ctla4
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: Please contact with the lab for this information.
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background: Cytotoxic T-lymphocyte protein 4 (CD152/CTLA4) is a ~24 kDa protein. Inhibitory receptor acting as a major negative regulator of T-cell responses. Acts as a decoy receptor: the affinity of CTLA4 for its natural B7 family ligands, CD80 and CD86, is considerably stronger than the affinity of their cognate stimulatory coreceptor CD28. CD152 is the therapeutic target of ipilimumab (Yervoy).
Note: For research use only.

Flow Cytometry

Flow-cytometry using anti-mouse CD152 antibody.CD152 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-mouse CD152 antibody monoclonal antibody (Catalog # MB651107 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Bioactivity

Detects recombinant mouse CD152/CTLA4 (Catalog No: PM10084) in indirect ELISA

Datasheet

$191.00