Anti-Human CD15/SSEA-1/Lewis x Antibody (SAA1433)
- Catalog number: FC10934
- Availability: In Stock
$191.00
- Ex Tax: $191.00
- Description: Anti-Human CD15/SSEA-1/Lewis x Antibody (SAA1433) [SAA1433] (FC10934) is a mouse monoclonal antibody detecting Human CD15/SSEA-1/Lewis x in ELISA, FCM, IHC. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
• Flow Cytometry — Validated for flow cytometry applications. - Model: HB132107
- Name: Anti-Human CD15/SSEA-1/Lewis x Antibody (SAA1433)
- Host species: Mouse
- Isotype: IgG2a, kappa
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4.
- Concentration: 1.32 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: ELISA, FCM, IHC
- Target: Alpha-(1,3)-fucosyltransferase 4, ELAM-1 ligand fucosyltransferase, Fucosyltransferase 4, Fucosyltransferase IV, Galactoside 3-L-fucosyltransferase, Fuc-TIV, FCT3A, FUT4, ELFT, FucT-IV
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: Alpha-(1,3)-fucosyltransferase 4 (CD15/SSEA-1) is a ~59 kDa protein. Catalyzes alpha(1->3) linkage of fucosyl moiety transferred from GDP-beta-L-fucose to N-acetyl glucosamine (GlcNAc) within type 2 lactosamine (LacNAc, Gal-beta(1->4)GlcNAc) glycan attached to N- or O-linked glycoproteins. Robustly fucosylates nonsialylated distal LacNAc unit of the polylactosamine chain to form Lewis X antigen (CD15), a glycan determinant known to mediate important cellular functions in development and immunity. Fucosylates with lower efficiency sialylated LacNAc acceptors to form sialyl Lewis X and 6-sulfo sialyl Lewis X determinants that serve as recognition epitopes for C-type lectins. Together with FUT7 contributes to SELE, SELL and SELP selectin ligand biosynthesis and selectin-dependent lymphocyte homing, leukocyte migration and blood leukocyte homeostasis. In a cell type specific manner, may also fucosylate the internal LacNAc unit of the polylactosamine chain to form VIM-2 antigen that serves as recognition epitope for SELE.
References:
1. Goelz, SE. et al. (1990) Cell 63, 1349-56. PMID: 1702034
2. Lowe, JB. et al. (1991) The Journal of biological chemistry 266, 17467-77. PMID: 171663
3. Mondal, N. et al. (2018) The Journal of biological chemistry 293, 7300-7314. PMID: 29593094
4. Nakayama, F. et al. (2001) The Journal of biological chemistry 276, 16100-6. PMID: 11278338 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Anti-Human CD15/SSEA-1/Lewis x Antibody (SAA1433)
