Anti-Human PODXL Antibody (Mab47)
- Catalog number: FC11011
- Availability: In Stock
$191.00
- Ex Tax: $191.00
- Description: Anti-Human PODXL Antibody (Mab47) [Mab47] (FC11011) is a mouse monoclonal antibody detecting PODXL in ELISA, FCM, IHC, Neutralization. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
• Flow Cytometry — Validated for flow cytometry applications. - Model: HT033107
- Name: Anti-Human PODXL Antibody (Mab47)
- Host species: Mouse
- Isotype: IgG2a, kappa
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4.
- Concentration: 2.37 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: ELISA, FCM, IHC, Neutralization
- Target: PCLP1, Podocalyxin-like protein 1, GCTM-2 antigen, Gp200, PODXL, Podocalyxin, PC, PCLP, PCLP-1
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: Podocalyxin (PODXL) is a ~58 kDa protein. Involved in the regulation of both adhesion and cell morphology and cancer progression. Functions as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis.
- Note: For research use only.
Images
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BioactivityDetects recombinant human PODXL (Cat No.: PH10053) in indirect ELISA.
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Western blotWestern blot analysis was performed using anti-PODXL monoclonal antibody at 1ug/mL on various samples.
Lane 1: recombinant human PODXL (Catalog No: PH10053)
Lane 2: negative control -
Flow cytometryFlow-cytometry using anti-human PODXL antibody. Hela cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human PODXL monoclonal antibody (Catalog HT033107, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
Datasheet
