Anti-Human CD27 Antibody (5F24)
- Catalog number: FC11023
- Availability: In Stock
$196.00
- Ex Tax: $196.00
- Description: Anti-Human CD27 Antibody (5F24) [5F24] (FC11023) is a mouse monoclonal antibody detecting CD27 in FCM, WB. Suitable for Human.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications. - Model: HB796207
- Name: Anti-Human CD27 Antibody (5F24)
- Host species: Mouse
- Isotype: IgG2a, kappa
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 0.09% Sodium azide.
- Concentration: 1 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: FCM, WB
- Target: CD27L receptor, TNFRSF7, T14, CD27 antigen, Tumor necrosis factor receptor superfamily member 7, CD27, T-cell activation antigen CD27
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: CD27 antigen is a ~29 kDa protein. Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue.
References:
1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863
2. Jaeger-Ruckstuhl, CA. et al. (2024) Immunity 57, 287-302.e12. PMID: 38354704 - Note: For research use only.
Images
-
Flow CytoMetryFlow-cytometry using anti-human CD27 antibody. Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CD27 monoclonal antibody (Catalog: HB796207) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a Goat Anti-Mouse IgG H&L Polyclonal Antibody, FITC (abinScience: MF690414) and cells analysed on a NovoCyte Flow Cytometer.
-
Western blotWestern blot analysis was performed using anti-CD27 monoclonal antibody at 1µg/ml on various samples.
Lane 1: recombinant human CD27 (Catalog No: PH11318)
Lane 2: negative control
Datasheet
