Anti-Human EGFR/ERBB1/HER1 Antibody (11F8)
- Catalog number: FC11039
- Availability: In Stock
$163.00
- Ex Tax: $163.00
- Description: Anti-Human EGFR/ERBB1/HER1 Antibody (11F8) [11F8] (FC11039) is a mouse monoclonal antibody detecting ERBB1 in ELISA, FCM. Suitable for Human.
Highlights:
• Flow Cytometry — Validated for flow cytometry applications. - Model: HF004307
- Name: Anti-Human EGFR/ERBB1/HER1 Antibody (11F8)
- Host species: Mouse
- Isotype: IgG2a, kappa
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 0.09% Sodium azide.
- Concentration: 1 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: ELISA, FCM
- Target: Epidermal growth factor receptor, 2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1, EGFR, ERBB, ERBB1, HER1
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: Epidermal growth factor receptor (EGFR/ERBB1) is a ~134 kDa protein. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses. Known ligands include EGF, TGFA/TGF-alpha, AREG, epigen/EPGN, BTC/betacellulin, epiregulin/EREG and HBEGF/heparin-binding EGF. Ligand binding triggers receptor homo- and/or heterodimerization and autophosphorylation on key cytoplasmic residues. The phosphorylated receptor recruits adapter proteins like GRB2 which in turn activates complex downstream signaling cascades. Activates at least 4 major downstream signaling cascades including the RAS-RAF-MEK-ERK, PI3 kinase-AKT, PLCgamma-PKC and STATs modules. EGFR is the therapeutic target of cetuximab (Erbitux) and panitumumab (Vectibix).
References:
1. Arcaro, A. et al. (2000) Molecular and cellular biology 20, 3817-30. PMID: 10805725
2. Runkle, KB. et al. (2016) Molecular cell 62, 385-396. PMID: 27153536
3. Chen, WS. et al. (1989) Cell 59, 33-43. PMID: 279096
4. Bu, J. et al. (2022) Acta biochimica et biophysica Sinica 54, 400-408. PMID: 35538033
5. Garrett, TP. et al. (2002) Cell 110, 763-73. PMID: 12297049
6. Kochupurakkal, BS. et al. (2005) The Journal of biological chemistry 280, 8503-12. PMID: 15611079
7. Kim, J. et al. (2007) Cancer research 67, 9229-37. PMID: 17909029
8. Lu, C. et al. (2010) Molecular and cellular biology 30, 5432-43. PMID: 20837704 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Anti-Human EGFR/ERBB1/HER1 Antibody (11F8)
