Anti-Human CD3 Antibody (HIT3a)
- Catalog number: FC11044
- Availability: In Stock
$196.00
- Ex Tax: $196.00
- Description: Anti-Human CD3 Antibody (HIT3a) [HIT3a] (FC11044) is a mouse monoclonal antibody detecting T-cell surface glycoprotein CD3 epsilon chain in ELISA, FCM, SPR. Suitable for Human.
Highlights:
• Multi-Application — Validated across multiple applications.
• Flow Cytometry — Validated for flow cytometry applications. - Model: HY057907
- Name: Anti-Human CD3 Antibody (HIT3a)
- Host species: Mouse
- Isotype: IgG2a, kappa
- Conjugation: Unconjugated
- Species reactivity: Human
- Form: Liquid
- Storage buffer: 0.01M PBS, pH 7.4, 0.09% Sodium azide.
- Concentration: 1 mg/ml
- Purity: >95% as determined by SDS-PAGE.
- Clonality: Monoclonal
- Applications: ELISA, FCM, SPR
- Target: T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e, CD3E, T3E
- Purification: Protein A/G purified from cell culture supernatant.
- Endotoxin level: Please contact with the lab for this information.
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
- Background: T-cell surface glycoprotein CD3 epsilon chain is a ~23 kDa protein. Part of the TCR-CD3 complex present on T-lymphocyte cell surface that plays an essential role in adaptive immune response. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD247/CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain. Upon TCR engagement, these motifs become phosphorylated by Src family protein tyrosine kinases LCK and FYN, resulting in the activation of downstream signaling pathways. CD3E ITAM phosphorylation creates docking sites for the protein kinase ZAP70 leading to ZAP70 phosphorylation and its conversion into a catalytically active enzyme.
References:
1. Gimferrer, I. et al. (2004) Journal of immunology (Baltimore, Md. : 1950) 173, 2262-70. PMID: 15294938
2. de Saint Basile, G. et al. (2004) The Journal of clinical investigation 114, 1512-7. PMID: 15546002
3. Barber, EK. et al. (1989) Proceedings of the National Academy of Sciences of the United States of America 86, 3277-81. PMID: 2470098
4. Du, J. et al. (2025) Cell 188, 4025-4042.e20. PMID: 40592325
5. Soudais, C. et al. (1993) Nature genetics 3, 77-81. PMID: 8490660 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Anti-Human CD3 Antibody (HIT3a)
Datasheet
