3C Protease
- Catalog number: EN10007
- Availability: In Stock
$311.00
- Ex Tax: $311.00
- Description: 3C protease is a cysteine protease that recognizes the cleavage site of Leu-Glu-Val-Leu-Phe-Gln*Gly-Pro.It used to remove fusion tags from proteins with the HRV 3C cleavage sequence and is dual tagged for easy removal from the sample after cleavage.
- Form: Liquid
- Storage buffer: 50mM Tris-HCl, pH 8.0, 150mM NaCl, 10mM EDTA, 1mM DTT, 50% glycerol
- Purity: >95% as determined by SDS-PAGE.
- Applications: Remove fusion tags from proteins with the HRV 3C cleavage sequence(LEVLFQ↓GP).
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E.coli
- Protein length: 3C Protease is cloned from Human rhinovirus and expressed in E.coli.
- Nature: Recombinant
- Predicted molecular weight: 47.46 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
- Species: HRV-14
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Note: For research use only.
Images
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SDS-PAGESDS-PAGE for 3C Protease
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BioactivityM: Marker
1: Control
2: Fusion protein digest with 3C proteaseCase study Formula concentration 1 mg/ml c mg/ml volume 100 µl V ml substrate 100 µg cV mg 10X Buffer 11 µl V/9 ml 3C Protease 1 µl cV/100 ml Mix the components thoroughly and incubate the reaction at 4°C for 16 hours. After incubation, take 2–10 µg of the control and digested sample for analysis by SDS-PAGE.
Datasheet
