Human CFB Recombinant Protein (N-His)
- Catalog number: PH10374
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human CFB Protein, N-His (PH10374) expressed in E. coli, spanning Tyr573-Leu761. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Tyr573-Leu761
- Predicted molecular weight: 23.77 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Properdin factor B, Complement factor B, GBG, Glycine-rich beta glycoprotein, BFD, C3/C5 convertase, CFB, PBF2, BF
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Complement factor B (CFB) is a ~85 kDa protein. Precursor of the catalytic component of the C3 and C5 convertase complexes of the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. The alternative complement pathway acts as an amplification loop that enhances other complement pathways (classical, lectin and GZMK) by promoting formation of additional C3 and C5 convertases. CFB is cleaved and activated by CFD to generate Ba and Bb chains; Bb chain constituting the catalytic component of the C3 and C5 convertases.
References:
1. Pangburn, MK. et al. (1986) The Biochemical journal 235, 723-30. PMID: 3638964
2. Vogt, W. et al. (1978) Immunology 34, 29-40. PMID: 624565
3. DiScipio, RG. et al. (1983) The Journal of biological chemistry 258, 10629-36. PMID: 6554279
4. Vogel, CW. et al. (1982) The Journal of biological chemistry 257, 8292-9. PMID: 6919543
5. Hourcade, DE. et al. (1998) The Journal of biological chemistry 273, 25996-6000. PMID: 9748277
6. Niemann, MA. et al. (1980) Biochemistry 19, 1576-83. PMID: 6769474 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human CFB Protein, N-His
