Human C7 Recombinant Protein (N-His)
- Catalog number: PH10842
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human C7 Protein, N-His (PH10842) expressed in E. coli, spanning Ser122-His456. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Ser122-His456
- Predicted molecular weight: 40.65 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: C7, Complement component C7
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Complement component C7 (Complement C7) is a ~93 kDa protein. Component of the membrane attack complex (MAC), a multiprotein complex activated by the complement cascade, which inserts into a target cell membrane and forms a pore, leading to target cell membrane rupture and cell lysis. The MAC is initiated by proteolytic cleavage of C5 into complement C5b in response to the classical, alternative, lectin and GZMK complement pathways. The complement pathways consist in a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. C7 serves as a membrane anchor. During MAC assembly, associates with C5b and C6 to form the C5b-7 complex, a key lipophilic precursor of the MAC complex, which associates with the outer leaflet and reduces the energy for membrane bending.
References:
1. Hadders, MA. et al. (2012) Cell reports 1, 200-7. PMID: 22832194
2. Serna, M. et al. (2016) Nature communications 7, 10587. PMID: 26841837
3. Sharp, TH. et al. (2016) Cell reports 15, 1-8. PMID: 27052168
4. Menny, A. et al. (2018) Nature communications 9, 5316. PMID: 30552328
5. DiScipio, RG. et al. (1988) The Journal of biological chemistry 263, 549-60. PMID: 3335508
6. Donado, CA. et al. (2025) Nature 641, 211-221. PMID: 39914456
7. Lan, F. et al. (2025) Nature 638, 490-498. PMID: 39814882 - Note: For research use only.
Images
-
SDS-PAGESDS-PAGE for Recombinant Human C7 Protein, N-His
Datasheet
