Human PKM Recombinant Protein (N-His)
- Catalog number: PH10974
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human PKM Protein, N-His (PH10974) expressed in E. coli, spanning Met1-Pro531. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Met1-Pro531
- Predicted molecular weight: 60.25 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: PK2, Opa-interacting protein 3, THBP1, Tumor M2-PK, PKM, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1, PK3, Pyruvate kinase 2/3, p58, CTHBP, PKM2, Pyruvate kinase PKM, OIP-3, Cytosolic thyroid hormone-binding protein, OIP3
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Pyruvate kinase PKM is a ~57 kDa protein. Catalyzes the final rate-limiting step of glycolysis by mediating the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.
References:
1. Dombrauckas, JD. et al. (2005) Biochemistry 44, 9417-29. PMID: 15996096
2. Ashizawa, K. et al. (1991) Biochemistry 30, 7105-11. PMID: 1854723
3. Vander Heiden, MG. et al. (2010) Science (New York, N.Y.) 329, 1492-9. PMID: 20847263 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human PKM Protein, N-His
