Human TSHR Recombinant Protein (N-His)
- Catalog number: PH11045
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human TSHR Protein, N-His (PH11045) expressed in E. coli, spanning Met1-Ser253. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Met1-Ser253
- Predicted molecular weight: 28.44 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution.
- Alternative Names: Thyroid-stimulating hormone receptor, TSH-R, TSHR, Thyrotropin receptor, LGR3
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Thyrotropin receptor (TSHR/LGR3) is a ~86 kDa protein. Receptor for the thyroid-stimulating hormone (TSH) or thyrotropin. Also acts as a receptor for the heterodimeric glycoprotein hormone (GPHA2:GPHB5) or thyrostimulin. TSHR is coupled to G(s) proteins and mediates the activation of adenylate cyclase. This leads to the generation of cyclic adenosine monophosphate (cAMP), which in turn activates protein kinase A (PKA). PKA subsequently phosphorylates downstream targets involved in thyroid hormone biosynthesis and secretion, including thyroid peroxidase (TPO) and the sodium/iodide symporter (NIS).
References:
1. Costagliola, S. et al. (2002) The EMBO journal 21, 504-13. PMID: 11847099
2. Nakabayashi, K. et al. (2002) The Journal of clinical investigation 109, 1445-52. PMID: 12045258
3. Faust, B. et al. (2022) Nature 609, 846-853. PMID: 35940205
4. Duan, J. et al. (2022) Nature 609, 854-859. PMID: 35940204
5. Morgan, SJ. et al. (2016) Thyroid : official journal of the American Thyroid Association 26, 1794-1803. PMID: 27638195 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human TSHR Protein, N-His
