Human CD27 Recombinant Protein (C-Fc)
- Catalog number: PH11318
- Availability: In Stock
$298.00
- Ex Tax: $298.00
- Description: Recombinant Human CD27 Protein, C-Fc (PH11318) expressed in Mammalian Cells, spanning Met1-Ile192.
Highlights:
• Fc Fusion — Enhanced stability with Protein A/G purification compatibility.
• Mammalian Expression — Proper folding and native glycosylation.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: Mammalian Cells
- Protein length: Met1-Ile192
- Predicted molecular weight: 47.84 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: CD27L receptor, TNFRSF7, T14, CD27 antigen, Tumor necrosis factor receptor superfamily member 7, CD27, T-cell activation antigen CD27
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: CD27 antigen is a ~29 kDa protein. Costimulatory immune-checkpoint receptor expressed at the surface of T-cells, NK-cells and B-cells which binds to and is activated by its ligand CD70/CD27L expressed by B-cells. The CD70-CD27 signaling pathway mediates antigen-specific T-cell activation and expansion which in turn provides immune surveillance of B-cells. Mechanistically, CD70 ligation activates the TRAF2-PTPN6 axis that subsequently inhibits LCK phosphorylation to promote phenotypic and transcriptional adaptations of T-cell memory. In addition, activation by CD70 on early progenitor cells provides a negative feedback signal to leukocyte differentiation during immune activation and thus modulates hematopoiesis. Negatively regulates the function of Th2 lymphocytes in the adipose tissue.
References:
1. Izawa, K. et al. (2017) The Journal of experimental medicine 214, 73-89. PMID: 28011863
2. Jaeger-Ruckstuhl, CA. et al. (2024) Immunity 57, 287-302.e12. PMID: 38354704 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human CD27 Protein, C-Fc
