Human STAT1 Recombinant Protein (N-His)
- Catalog number: PH11576
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human STAT1 Protein, N-His (PH11576) expressed in E. coli, spanning Met1-Arg683. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Met1-Arg683
- Predicted molecular weight: 82.25 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Signal transducer and activator of transcription 1-alpha/beta, STAT1, Transcription factor ISGF-3 components p91/p84
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Signal transducer and activator of transcription 1-alpha/beta (STAT1) is a ~87 kDa protein. Signal transducer and transcription activator that mediates cellular responses to interferons (IFNs), cytokine KITLG/SCF and other cytokines and other growth factors. Following type I IFN (IFN-alpha and IFN-beta) binding to cell surface receptors, signaling via protein kinases leads to activation of Jak kinases (TYK2 and JAK1) and to tyrosine phosphorylation of STAT1 and STAT2. The phosphorylated STATs dimerize and associate with ISGF3G/IRF-9 to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of IFN-stimulated genes (ISG), which drive the cell in an antiviral state. In response to type II IFN (IFN-gamma), STAT1 is tyrosine- and serine-phosphorylated.
References:
1. Rogers, RS. et al. (2003) The Journal of biological chemistry 278, 30091-7. PMID: 12764129
2. Ungureanu, D. et al. (2003) Blood 102, 3311-3. PMID: 12855578
3. DeVries, TA. et al. (2004) The Journal of biological chemistry 279, 45603-12. PMID: 15322115
4. Yeh, HM. et al. (2013) Journal of immunology (Baltimore, Md. : 1950) 191, 3328-36. PMID: 23940278
5. Li, M. et al. (2021) Experimental cell research 407, 112784. PMID: 34508746
6. Alphonse, N. et al. (2022) Cell 185, 2354-2369.e17. PMID: 35568036
7. Liu, B. et al. (1998) Proceedings of the National Academy of Sciences of the United States of America 95, 10626-31. PMID: 9724754
8. Chen, K. et al. (2017) Cell 170, 492-506.e14. PMID: 28753426
9. Zhang, Y. et al. (2015) Nature immunology 16, 1215-27. PMID: 26479788 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human STAT1 Protein, N-His
