Human NEK2 Recombinant Protein (N-His)
- Catalog number: PH11756
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human NEK2 Protein, N-His (PH11756) expressed in E. coli, spanning Lys125-Leu212. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Lys125-Leu212
- Predicted molecular weight: 12.36 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: NLK1, NEK2, NimA-like protein kinase 1, HSPK 21, NEK2A, Serine/threonine-protein kinase Nek2, NimA-related protein kinase 2, Never in mitosis A-related kinase 2
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Serine/threonine-protein kinase Nek2 is a ~51 kDa protein. Protein kinase which is involved in the control of centrosome separation and bipolar spindle formation in mitotic cells and chromatin condensation in meiotic cells. Regulates centrosome separation (essential for the formation of bipolar spindles and high-fidelity chromosome separation) by phosphorylating centrosomal proteins such as CROCC, CEP250 and NINL, resulting in their displacement from the centrosomes. Regulates kinetochore microtubule attachment stability in mitosis via phosphorylation of NDC80. Involved in regulation of mitotic checkpoint protein complex via phosphorylation of CDC20 and MAD2L1. Plays an active role in chromatin condensation during the first meiotic division through phosphorylation of HMGA2.
References:
1. Fang, G. et al. (2014) Journal of cell science 127, 1631-9. PMID: 24554434
2. Man, X. et al. (2015) European journal of cell biology 94, 162-72. PMID: 25704143
3. Chen, C. et al. (2015) Journal of cell science 128, 3290-303. PMID: 26220856
4. Xia, Y. et al. (2018) Journal of cell science 131. PMID: 30404835 - Note: For research use only.
Images
-
SDS-PAGESDS-PAGE for Recombinant Human NEK2 Protein, N-His
