Human PAK2 Recombinant Protein (N-His)
- Catalog number: PH12158
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human PAK2 Protein, N-His (PH12158) expressed in E. coli, spanning Glu177-Ala419. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Glu177-Ala419
- Predicted molecular weight: 29.16 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: S6/H4 kinase, p21-activated kinase 2, p58, PAK-2, PAK2, C-t-PAK2, PAK65, p34, Gamma-PAK, Serine/threonine-protein kinase PAK 2, p27
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Serine/threonine-protein kinase PAK 2 (PAK2) is a ~58 kDa protein. Serine/threonine protein kinase that plays a role in a variety of different signaling pathways including cytoskeleton regulation, cell motility, cell cycle progression, apoptosis or proliferation. Acts as a downstream effector of the small GTPases CDC42 and RAC1. Activation by the binding of active CDC42 and RAC1 results in a conformational change and a subsequent autophosphorylation on several serine and/or threonine residues. Full-length PAK2 stimulates cell survival and cell growth. Phosphorylates MAPK4 and MAPK6 and activates the downstream target MAPKAPK5, a regulator of F-actin polymerization and cell migration.
References:
1. Jakobi, R. et al. (2003) The Journal of biological chemistry 278, 38675-85. PMID: 12853446
2. Vilas, GL. et al. (2006) Proceedings of the National Academy of Sciences of the United States of America 103, 6542-7. PMID: 16617111
3. Mitsushima, M. et al. (2009) Molecular and cellular biology 29, 2816-27. PMID: 19273597
4. Hsu, RM. et al. (2010) Molecular biology of the cell 21, 287-301. PMID: 19923322
5. Antonarakis, SE. et al. (2021) Human molecular genetics 31, 1-9. PMID: 33693784
6. Martin, GA. et al. (1995) The EMBO journal 14, 1970-8. PMID: 7744004
7. Rudel, T. et al. (1997) Science (New York, N.Y.) 276, 1571-4. PMID: 9171063 - Note: For research use only.
Images
-
SDS-PAGESDS-PAGE for Recombinant Human PAK2 Protein, N-His.
