Anti-Human IL-18/IL-1F4 Reference Antibody (Humax-Il8, RUO)

Description: HuMax-IL8 (BS10532) is a research-grade recombinant antibody targeting GCP/IL-8 protein II. Produced in mammalian cells with native-like glycosylation.
Highlights:
• Research Grade — For PK/PD studies, assay development, and ADA research.
• Native Glycosylation — Mammalian expression ensures native-like patterns.
Host species: Human
Isotype: IgG1-kappa
Species reactivity: Human
Immunogen:
Form: Liquid
Storage instructions:
Storage buffer: 0.01M PBS, pH7.4.
Concentration: 2.6mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: Research Grade Biosimilar
Target: GCP/IL-8proteinII,MDNCF,IL8/NAP1formIII,IL-8(1-77),Neutrophil-activatingfactor,GCP/IL-8proteinI,GCP/IL-8proteinVI,Interleukin-8,Chemokine(C-X-Cmotif)ligand8,Neutrophil-activatingprotein1,Monocyte-derivedneutrophilchemotacticfactor,(Ser-IL-8)72,MDNCF-b,LYNAP,GCP/IL-8proteinV,IL8/NAP1formIV,MONAP,IL8/NAP1formV,IL8/NAP1formVI,NAP-1,Granulocytechemotacticprotein1,GCP/IL-8proteinIII,IL8/NAP1formII,IL8,IL-8,CXCL8,IL8/NAP1formI,(Ala-IL-8)77,Protein3-10C,GCP/IL-8proteinIV,Emoctakin,GCP-1,C-X-Cmotifchemokine8,NAF,T-cellchemotacticfactor,MDNCF-c,Monocyte-derivedneutrophil-activatingpeptide,Lymphocyte-derivedneutrophil-activatingfactor
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: <1EU/mg, determined by LAL gel clotting assay
Expression system: Mammalian Cells
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names: HuMab10F8,HuMax-IL8,MDX-018,formerlyHuMax-Inflam
Background: Interleukin-8 (IL8/CXCL8) is a ~11 kDa protein. Chemotactic factor that mediates inflammatory response by attracting neutrophils, basophils, and T-cells to clear pathogens and protect the host from infection. Also plays an important role in neutrophil activation. Released in response to an inflammatory stimulus, exerts its effect by binding to the G-protein-coupled receptors CXCR1 and CXCR2, primarily found in neutrophils, monocytes and endothelial cells. G-protein heterotrimer (alpha, beta, gamma subunits) constitutively binds to CXCR1/CXCR2 receptor and activation by IL8 leads to beta and gamma subunits release from Galpha (GNAI2 in neutrophils) and activation of several downstream signaling pathways including PI3K and MAPK pathways.
References:
1. Zinkernagel, AS. et al. (2008) Cell host & microbe 4, 170-8. PMID: 18692776
2. Hammond, ME. et al. (1995) Journal of immunology (Baltimore, Md. : 1950) 155, 1428-33. PMID: 7636208
3. Van Damme, J. et al. (1990) European journal of immunology 20, 2113-8. PMID: 2145175
4. Jacobi, HH. et al. (1998) International archives of allergy and immunology 116, 53-9. PMID: 962351
5. Holmes, WE. et al. (1991) Science (New York, N.Y.) 253, 1278-80. PMID: 1840701
6. Murphy, PM. et al. (1991) Science (New York, N.Y.) 253, 1280-3. PMID: 1891716
7. Takami, M. et al. (2002) Journal of immunology (Baltimore, Md. : 1950) 168, 4559-66. PMID: 11971003
8. Damaj, BB. et al. (1996) The Journal of biological chemistry 271, 12783-9. PMID: 8662698
Note: For research use only. Not suitable for clinical or therapeutic use.