Human DNMT3A Recombinant Protein (N-His)
- Catalog number: PH13152
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human DNMT3A Protein, N-His (PH13152) expressed in E. coli, spanning Ala628-Val912. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Ala628-Val912
- Predicted molecular weight: 34.98 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Dnmt3a, DNA MTase HsaIIIA, DNMT3A, M.HsaIIIA, DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: DNA (cytosine-5)-methyltransferase 3A (DNMT3A) is a ~101 kDa protein. Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting.
References:
1. Chen, T. et al. (2002) The Journal of biological chemistry 277, 38746-54. PMID: 12138111
2. Viré, E. et al. (2006) Nature 439, 871-4. PMID: 1635787
3. Heyn, P. et al. (2019) Nature genetics 51, 96-105. PMID: 30478443 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human DNMT3A Protein, N-His.
