Human LC3B/MAP1LC3B Recombinant Protein (N-His)
- Catalog number: PH13443
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human LC3B/MAP1LC3B Protein, N-His (PH13443) expressed in E. coli, spanning Met1-Gly120. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Met1-Gly120
- Predicted molecular weight: 16.44 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: MAP1A/MAP1B light chain 3 B, MAP1 light chain 3-like protein 2, MAP1ALC3, MAP1LC3B, Autophagy-related protein LC3 B, Microtubule-associated protein 1 light chain 3 beta, Microtubule-associated proteins 1A/1B light chain 3B, Autophagy-related ubiquitin-like modifier LC3 B, MAP1A/MAP1B LC3 B
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Microtubule-associated protein 1 light chain 3 beta (LC3B/MAP1LC3B) is a ~14 kDa protein. Ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). Plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. In response to cellular stress and upon mitochondria fission, binds C-18 ceramides and anchors autophagolysosomes to outer mitochondrial membranes to eliminate damaged mitochondria. While LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway.
References:
1. Weidberg, H. et al. (2010) The EMBO journal 29, 1792-802. PMID: 20418806
2. Zhu, Y. et al. (2013) The Journal of biological chemistry 288, 1099-113. PMID: 23209295
3. Wei, Y. et al. (2017) Cell 168, 224-238.e10. PMID: 28017329
4. Sentelle, RD. et al. (2012) Nature chemical biology 8, 831-8. PMID: 22922758
5. Tang, Z. et al. (2013) Nature 502, 254-7. PMID: 24089205
6. An, H. et al. (2019) Molecular cell 74, 891-908.e10. PMID: 31006537
7. Chino, H. et al. (2019) Molecular cell 74, 909-921.e6. PMID: 31006538 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human LC3B/MAP1LC3B Protein, N-His
