Anti-Human IL-1 beta/IL-1F2 Reference Antibody (Canakinumab, RUO)
- Catalog number: BS10584
- Availability: In Stock
$176.00
- Ex Tax: $176.00
Highlights:
• Research Grade — For PK/PD studies, assay development, and ADA research.
• Native Glycosylation — Mammalian expression ensures native-like patterns.
Host species: Human
Isotype: IgG1-kappa
Species reactivity: Human
Immunogen:
Form: Liquid
Storage instructions:
Storage buffer: 0.01M PBS, pH7.4.
Concentration: 1.94mg/ml
Purity: >95% as determined by SDS-PAGE.
Clonality: Monoclonal
Applications: Research Grade Biosimilar
Target: Interleukin-1beta,IL1B,Catabolin,IL1F2,IL-1beta
Purification: Protein A/G purified from cell culture supernatant.
Endotoxin level: <1EU/mg, determined by LAL gel clotting assay
Expression system: Mammalian Cells
Stability and Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names: ACZ885,CAS:914613-48-2
Background: Interleukin-1 beta (IL1B/IL1F2) is a ~30 kDa protein. Potent pro-inflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6. IL1B is the therapeutic target of canakinumab (Ilaris).
References:
1. Tominaga, K. et al. (2000) International immunology 12, 151-60. PMID: 1065385
2. Nakahara, H. et al. (2003) Arthritis and rheumatism 48, 1521-9. PMID: 12794819
3. LaRock, CN. et al. (2016) Science immunology 1. PMID: 28331908
4. Van Damme, J. et al. (1985) Nature 314, 266-8. PMID: 3920526
5. Tian, XX. et al. (2021) International endodontic journal 54, 916-925. PMID: 33377178
6. Xia, S. et al. (2021) Nature 593, 607-611. PMID: 33883744
Note: For research use only. Not suitable for clinical or therapeutic use.
Images
-
BioactivityDetects Human IL1B/IL1F2 in indirect ELISAs.
-
Flow cytometryFlow-cytometry using PE anti-human IL1B antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an PE anti-human IL1B monoclonal antibody (Catalog HF943016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
-
Flow cytometryFlow-cytometry using anti-human IL1B antibody. 5637 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an anti-human IL1B monoclonal antibody (Catalog HF943016, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Goat Anti-Human IgG H&L Polyclonal Antibody, FITC (abinScience: HF690414) and cells analysed on a NovoCyte Flow Cytometer.
