Human CFD Recombinant Protein (C-His)
- Catalog number: PH14988
- Availability: In Stock
$298.00
- Ex Tax: $298.00
- Description: Recombinant Human CFD Protein, C-His (PH14988) expressed in Mammalian Cells, spanning Met1-Ala253.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• Mammalian Expression — Proper folding and native glycosylation.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: Mammalian Cells
- Protein length: Met1-Ala253
- Predicted molecular weight: 28.00 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution.A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Complement factor D, 3.4.21.46, Adipsin, C3 convertase activator, Properdin factor D, CFD, DF, PFD
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Complement factor D (CFD) is a ~27 kDa protein. Serine protease that initiates the alternative pathway of the complement system, a cascade of proteins that leads to phagocytosis and breakdown of pathogens and signaling that strengthens the adaptive immune system. In contrast to other complement pathways (classical, lectin and GZMK) that are directly activated by pathogens or antigen-antibody complexes, the alternative complement pathway is initiated by the spontaneous hydrolysis of complement C3. The alternative complement pathway acts as an amplification loop that enhances complement activation by mediating the formation of C3 and C5 convertases. Activated CFD cleaves factor B (CFB) when the latter is complexed with complement C3b, activating the C3 convertase of the alternative pathway.
References:
1. Forneris, F. et al. (2010) Science (New York, N.Y.) 330, 1816-20. PMID: 21205667
2. Katschke, KJ. et al. (2012) The Journal of biological chemistry 287, 12886-92. PMID: 22362762
3. Niemann, MA. et al. (1980) Biochemistry 19, 1576-83. PMID: 6769474
4. Volanakis, JE. et al. (1977) Journal of immunology (Baltimore, Md. : 1950) 119, 337-42. PMID: 874324
5. Hourcade, DE. et al. (1998) The Journal of biological chemistry 273, 25996-6000. PMID: 9748277 - Note: For research use only.
Images
-
SDS-PAGESDS-PAGE for Recombinant Human CFD Protein, C-His
