Human AKR1B1 Recombinant Protein (N-His)
- Catalog number: PH15030
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human AKR1B1 Protein, N-His (PH15030) expressed in E. coli, spanning Met1-Phe316. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Met1-Phe316
- Predicted molecular weight: 38.02 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: Aldo-keto reductase family 1 member B1, 1.1.1.300, 1.1.1.372, 1.1.1.54, Aldehyde reductase, Aldose reductase, AR, 1.1.1.21, AKR1B1, ALDR1, ALR2
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Aldo-keto reductase family 1 member B1 (AKR1B1) is a ~35 kDa protein. Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols. Displays enzymatic activity towards endogenous metabolites such as aromatic and aliphatic aldehydes, ketones, monosacharides, bile acids and xenobiotics substrates. Key enzyme in the polyol pathway, catalyzes reduction of glucose to sorbitol during hyperglycemia. Reduces steroids and their derivatives and prostaglandins. Displays low enzymatic activity toward all-trans-retinal, 9-cis-retinal, and 13-cis-retinal.
References:
1. Hamada, Y. et al. (1991) Diabetes 40, 1233-40. PMID: 1936586
2. Crosas, B. et al. (2003) The Biochemical journal 373, 973-9. PMID: 12732097
3. Kabututu, Z. et al. (2009) Journal of biochemistry 145, 161-8. PMID: 19010934
4. Liu, SQ. et al. (1993) Biochimica et biophysica acta 1164, 268-72. PMID: 8343525
5. Spite, M. et al. (2007) The Biochemical journal 405, 95-105. PMID: 17381426
6. Shen, Y. et al. (2011) Chemico-biological interactions 191, 192-8. PMID: 21329684 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human AKR1B1 Protein, N-His
