Human LBR Recombinant Protein (N-GST & C-His)
- Catalog number: PH15086
- Availability: In Stock
$211.00
- Ex Tax: $211.00
- Description: Recombinant Human LBR Protein, N-GST & C-His (PH15086) expressed in E. coli, spanning Ala7-Arg61. Purity: >90% by SDS-PAGE.
Highlights:
• His-Tagged — N-terminal 6×His tag for IMAC purification.
• E. coli Expression — High-yield, cost-effective production.
• High Purity — >90% purity verified by SDS-PAGE. - Form: Lyophilized
- Storage buffer: Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
- Purity: >90% as determined by SDS-PAGE.
- Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
- Endotoxin level: Please contact with the lab for this information.
- Expression system: E. coli
- Protein length: Ala7-Arg61
- Predicted molecular weight: 34.50 kDa
- Stability and Storage: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
- Reconstitution: Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
- Alternative Names: LMN2R, LBR, C14SR, Integral nuclear envelope inner membrane protein, Sterol C14-reductase, 3-beta-hydroxysterol Delta (14)-reductase, Delta(14)-sterol reductase LBR, Lamin-B receptor, Delta-14-SR, C-14 sterol reductase
- Species: Homo sapiens (Human)
- Shipping: In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
- Background: Delta(14)-sterol reductase LBR is a ~70 kDa protein. Catalyzes the reduction of the C14-unsaturated bond of lanosterol, as part of the metabolic pathway leading to cholesterol biosynthesis. Plays a critical role in myeloid cell cholesterol biosynthesis which is essential to both myeloid cell growth and functional maturation. Mediates the activation of NADPH oxidases, perhaps by maintaining critical levels of cholesterol required for membrane lipid raft formation during neutrophil differentiation. Anchors the lamina and the heterochromatin to the inner nuclear membrane.
References:
1. Waterham, HR. et al. (2003) American journal of human genetics 72, 1013-7. PMID: 12618959
2. Bennati, AM. et al. (2006) Biochimica et biophysica acta 1761, 677-85. PMID: 16784888
3. Clayton, P. et al. (2010) Nucleus (Austin, Tex.) 1, 354-66. PMID: 21327084
4. Tsai, PL. et al. (2016) eLife 5. PMID: 27336722
5. Silve, S. et al. (1998) Biochimica et biophysica acta 1392, 233-44. PMID: 963065
6. Duband-Goulet, I. et al. (2000) Biochemistry 39, 6483-8. PMID: 10828963 - Note: For research use only.
Images
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SDS-PAGESDS-PAGE for Recombinant Human LBR Protein, N-GST & C-His
